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The membrane was incubated with Anti-mouse IgG, HRP-linked secondary antibody, 7076), Anti-rabbit IgG, HRP-linked secondary antibody (Cell Signaling Technology, 7074) followed by chemiluminescence detection (Thermo scientific, 34080, and BIO-RAD, 1705061) with the ChemiDocTM Touch Imaging System (BIO-RAD, California, USA)